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Detection and quantification of viral and satellite RNAs in plant hosts

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Northern blotting is a valuable method for detection and quantification of RNA in the field of virology. Although many methods including a various versions of polymerase chain reaction have been developed over the years, Northern blotting has been still considered as a useful and effective method for the analysis of progeny RNA accumulation for viral and subviral pathogens, such as satellite RNAs, in plant hosts. Here, we describe a detailed Northern blot protocol for efficient detection and quantification of viral and satellite RNAs from plant hosts.

Original languageEnglish
Title of host publicationRNA Abundance Analysis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages131-141
Number of pages11
ISBN (Print)9781617798382
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume883
ISSN (Print)1064-3745

Keywords

  • Northern blot
  • RNA detection and quantification
  • Satellite RNA
  • Viral RNA

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