Abstract
Tomato chlorosis virus (ToCV), a member of the genus Crinivirus, has caused an epidemic disease in tomato worldwide. ToCV is phloem-limited and transmitted by whiteflies in a semi-persistent manner, but not by mechanical inoculation. Experimental propagation of ToCV has been performed primarily by using whitefly-mediated inoculation. To develop a simple and convenient method for transmission of ToCV, we investigated grafting single-leaflets from tomato plants infected with ToCV to recipient tomato seedlings. Forty-one of 46 tomato seedlings tested were grafted successfully with single-leaflets infected with ToCV. Among them, 36 seedlings (87.8%) were systemically infected with ToCV and developed typical symptoms. Our results demonstrated that single-leaflet grafting could provide a sufficient amount of inoculum for the transmission of ToCV to the grafted seedlings.
| Original language | English |
|---|---|
| Pages (from-to) | 345-349 |
| Number of pages | 5 |
| Journal | Plant Pathology Journal |
| Volume | 33 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2017 |
Bibliographical note
Publisher Copyright:© The Korean Society of Plant Pathology.
Keywords
- Graft inoculation
- Single-leaflet
- Tomato chlorosis virus